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Tag: Medicine

A Healthier Alternative to Antibiotics

New study proves biological treatment can be a suitable alternative to antibiotics.

In a groundbreaking new study led by Dr. Natalia Freund and doctoral candidate Avia Watson at the TAU Sackler Faculty of Medicine, researchers were able to develop a “biological antibiotic” and demonstrates that human antibodies can offer an alternative to the traditional chemical antibiotics. The study was conducted in collaboration with laboratories in the United States and China and published in the prestigious scientific journal Nature Communications.

During the past century, antibiotics have served as the main treatment against bacteria, being both efficient and cheap. Antibiotics are chemical agents, designed to block and destroy specific cells, such as microbial cells. However, since some biological mechanisms are common to both human and microbial cells, the range of antibiotics that can safely be used without harming the patient is limited. For example, cell wall components of many strains of microbes are common to human cells; therefore, any damage caused to the microbial cell walls can lead to extensive damage to body systems. Furthermore, in recent years the number of microbial strains that are resistant to existing antibiotics has grown, which presents new challenges of defending the body from microbes in the post-antibiotic era.

For these reasons, Dr. Natalia Freund and her laboratory team have spent the recent years searching for a biological alternative to known antibiotics. Dr. Freund explains, “Advances in biological medicine have enabled us to rout the germs in new ways that are not based solely on antibiotics, allowing for a solution to the challenge posed by resistant germs. Our study is an initial proof of the concept of employing monoclonal antibodies (derived from single cells) as an effective therapy for combating bacterial pathogens”. Antibodies are proteins that are produced naturally by our immune response following infection or a vaccine. They harbor many advantages such as specificity, stability and safety. This is why antibodies are today in widespread use in the clinic for treatment of cancer, autoimmune diseases and viral infections such as COVID-19.

Tuberculosis as Test Case

The research team chose Tuberculosis, which is caused by infection of the bacilli Mycobacterium tuberculosis, as a test case and were able, for the first time ever, to create an effective treatment based on anti-bacterial antibodies that developed naturally during infection (the antibodies were extracted from a patient who had been infected, and has since recovered, from tuberculosis). Another reason for the choice of tuberculosis is that although the vaccine against tuberculosis was developed 100 years ago (and is based on the attenuated bacillus bovis (BCG) strain), it is not effective for adults and does not prevent infection. In addition, in recent years, more and more strains of disease have developed that are resistant to the only treatment currently available: treatment with antibiotics. Since tuberculosis bacteria are highly contagious and are transmitted through the air and damaging to the lungs, the spread of untreated resistant strains of tuberculosis constitutes a real hazard. Today, about a quarter of the world’s population is infected with tuberculosis, with the rates of drug-resistant strains peaking   as high as 40% in some countries. In Israel, there are about 200 active tuberculosis cases every year.

 

Dr. Natalia Freund and her research team

Future Targets: Pneumonia and Staphylococcal Infections

Due to the size and complexity of the tuberculosis bacillus, previous efforts to isolate monoclonal antibodies against it have been futile. The researchers in Dr. Freund’s laboratory have succeeded in isolating two types of antibodies which contributed to a 50% reduction of the bacterial levels in mice relative to other mice that were not treated with antibodies. These antibodies have been found to be effective against three different strains of the tuberculosis bacterium and are expected to be effective also against additional strains that have not yet been investigated, including strains that are resistant to antibiotics

Following the success of the study, Dr. Freund’s laboratory is currently exploring the possibility of extending the “biological” substitute for antibiotics to include other diseases. “The demonstrated case for this study will enable us to expand on our future work to include diseases such as pneumonia and staphylococcal infections,” says Dr. Freund.

Children with Autism during Lockdown: Serious Implications for Behavior and Development

A new TAU study examines the difficulties experienced by children with different levels of autism and their parents during Israel’s first lockdown during the coronavirus crisis (in the spring of 2020). The data shows that the drastic changes in the children’s routines and their prolonged stay in their homes instead of their special education had serious implications for the behavior and development of the children and impacted their parent’s ability to support them

The study was led by Dr. Itay Tokatly-Latzer, Prof. Orit Karnieli-Miller and Prof. Yael Leitner from the TAU Sackler Faculty of Medicine, in collaboration with the Tel-Aviv Sourasky Medical Center, and was published in the academic journal ‘Autism’.

Lack of Routine Caused Regression

The study group consisted of the parents of 25 children with autism who shared the difficulties they faced during lockdown with the researchers in real time. Some children would for instance refuse to go to sleep at night – screaming and restless, they would stay awake all through the night. Others experienced behavioral regression, returning to repetitive and stereotypical movements that had initially improved.

At the same time, however, the researchers note that there were families who experienced it differently and who found creative ways to help their children get through the crisis in a positive way: One couple chose to go along with the particular interests of their child, engaging in repetitive cake baking. The parents of another child who needed to be in constant movement, bought their son a trampoline so that he could spend his excess energy inside their home.

Support and Guidance Programs for Parents of Children with Autism

Prof. Karnieli-Miller: “Lockdowns are difficult for all of us, but all the more so for families with autistic children. For these children, even the slightest change of routine can cause harm and throw them off balance. The study showed that in many instances the parents were left helpless as they did not have the tools and the professional knowledge required to deal with the situation. The parents need to be given the tools, support and guidance in order to deal with this huge challenge and enable them to create a ‘flexible routine’ for their children.

“The findings of the study show that during periods of lockdowns the State must do whatever it takes to prevent the closing down of special education, in order to prevent causing harm to children with special needs. If the State nevertheless decides that such steps be taken, it must immediately implement assistance and guidance programs for parents of children with autism. The parents need to receive professional help and better tools for caring for their children.”

Cancer Breakthrough: Cells’ Uniqueness is Also Weakness

TAU research proves connection for first time, can be base for cancer drugs.

What makes cancer cells different from ordinary cells in our bodies? Can these differences be used to strike at them and paralyze their activity? This basic question has bothered cancer researchers since the mid-19th century. The search for unique characteristics of cancer cells is a building block of modern cancer research. A new study led by researchers from Tel Aviv University shows, for the first time, how an abnormal number of chromosomes (aneuploidy) — a unique characteristic of cancer cells that researchers have known about for decades — could become a weak point for these cells. The study could lead, in the future, to the development of drugs that will use this vulnerability to eliminate the cancer cells.

The study, which was published in Nature, was conducted in the laboratory of Dr. Uri Ben-David of the Sackler Faculty of Medicine at Tel Aviv University, in collaboration with six laboratories from four other countries (the United States, Germany, the Netherlands, and Italy).

Aneuploidy is a hallmark of cancer. While normal human cells contain two sets of 23 chromosomes each — one from the father and one from the mother — aneuploid cells have a different number of chromosomes. When aneuploidy appears in cancer cells, not only do the cells “tolerate” it, but it can even advance the progression of the disease. The relationship between aneuploidy and cancer was discovered over a century ago, long before it was known that cancer was a genetic disease (and even before the discovery of DNA as hereditary material).

According to Dr. Ben-David, aneuploidy is actually the most common genetic change in cancer. Approximately 90% of solid tumors, such as breast cancer and colon cancer, and 75% of blood cancers, are aneuploid. However, our understanding of the manner in which aneuploidy contributes to the development and spread of cancer is limited.

In the study, the researchers used advanced bioinformatic methods to quantify aneuploidy in approximately 1,000 cancer cell cultures. Then, they compared the genetic dependency and drug sensitivity of cells with a high level of aneuploidy to those of cells with a low level of aneuploidy. They found that aneuploid cancer cells demonstrate increased sensitivity to inhibition of the mitotic checkpoint – a cellular checkpoint that ensures the proper separation of chromosomes during cell division.

They also discovered the molecular basis for the increased sensitivity of aneuploid cancer cells. Using genomic and microscopic methods, the researchers tracked the separation of chromosomes in cells that had been treated with a substance that is known to inhibit the mitotic checkpoint. They found that when the mitotic checkpoint is perturbed in cells with the proper number of chromosomes, cell division stops. As a result, the chromosomes in the cells separate successfully, and relatively few chromosomal problems are created. But when this mechanism is perturbed in aneuploid cells, cell division continues, resulting in the creation of many chromosomal changes that compromise the cells’ ability to divide, and even cause their death.

The study has important implications for the drug discovery process in personalized cancer medicine. Drugs that delay the separation of chromosomes are undergoing clinical trials, but it is not known which patients will respond to them and which will not. The results of this study suggest that it will be possible to use aneuploidy as a biological marker, based on possibility to find the patients who will respond better to these drugs. To put it another way, it will be possible to adapt drugs that are already in clinical trials for use against tumors with specific genetic characteristics.

In addition, the researchers propose focusing the development of new drugs on specific components of the mechanism of chromosomal separation, which were identified as especially critical to aneuploid cancer cells. The mitotic checkpoint is made up of several proteins. The study shows that the aneuploid cells’ sensitivity to inhibition of the various proteins is not identical, and that some proteins are more essential to cancer cells than others. Therefore, the study provides motivation for developing specific drugs against additional proteins in the mitotic checkpoint.

“It should be emphasized that the study was done on cells in culture and not on actual tumors, and in order to translate it to treatment of cancer patients, many more follow-up studies must be conducted. If they hold true in patients, however, our findings would have a number of important medical implications,” Dr. Ben-David says.

The study was conducted in collaboration with laboratories from five countries: Dr. Zuzana Storchová, (Technische Universität Kaiserslautern, Germany), Dr. Jason Stumpff (University of Vermont, USA), Dr. Stefano Santaguida (University of Milano, Italy), Dr. Floris Foijer (University of Groningen, the Netherlands), and Dr. Todd Golub (The Broad Institute of MIT and Harvard, USA).

Academic First Responders

How TAU sparked a learning revolution in the wake of COVID-19.

By Idit Nirel

When COVID-19 broke in Israel in mid-March and the country shut down, Tel Aviv University (TAU) decided to continue teaching all courses online—almost overnight.

While many professors and students struggled to adapt, Prof. Guy Mundlak was ready.  

Prof. Guy Mundlak

​Prof. Guy Mundlak. Photo: Yoram Reshef.

Mundlak, who teaches both at the Buchmann Faculty of Law and the Department of Labor Studies of the Gordon Faculty of Social Sciences, made the change to online teaching 4 years ago. One of his courses, “Labor Law,” is a hybrid course; students study theoretical materials on their own through online videos of lectures, and the in-person sessions are dedicated to discussions and analyzing the latest case studies. Mundlak’s motivation to go digital preceded COVID-19 and stemmed from a different reason:

“Teaching this course for over 20 years, I couldn’t reinvent the wheel and find new ways to teach the same material every time,” he says. Making the course digital refreshed it.

Mundlak sees online learning not as a constraint, but as an opportunity: “The format allows students to learn the general concepts at their own pace, and I can focus my classroom lessons on what interests us here and now, without worrying if I’ve covered all of the material in time for the exam,” he explains. “This approach leaves me more room for spontaneity, for dealing with matters of the hour, and for diving deep into topics with the students. As a result, I don’t just lecture to my students; I engage and involve them in issues that touch their everyday lives—which is the best way to learn.”

With the pandemic and lockdown crushing the economy, Mundlak’s course became especially relevant to his students in the spring of 2020. He dedicated his classes—taught via Zoom—to employment issues that emerged during the Corona pandemic, such as the ramifications of layoffs and furloughs. Because most of his students had been working as waiters or in other hourly jobs to finance their studies, these subjects were not just academic theory, but reality, for many of them.

Coronavirus Pushes Learning Online

Dr. Tal Soffer. Photo: Yoram Reshef.

Providing Prof. Mundlak with digital tools for online teaching was Dr. Tal Soffer, Director of Virtual TAU, the unit responsible for enhancing the University’s digital teaching capacity and resources.  According to her, “online courses or integrating digital methods into other courses allow for learning that is customized to students’ needs.” At the same time, “online learning can provide students with skills for lifelong learning, which are crucial for success in today’s labor market—such as time management and the ability to learn independently.”

As Coronavirus spread in Israel and lockdown appeared imminent, Soffer and her team were already working around the clock to facilitate the shift to online studies. It was a success. More than 90,000 live online lessons took place over the spring semester, in addition to thousands of lessons recorded for independent study. All in all, online learning during the lockdown accounted for more than 50,000 hours and 10 Terabits in digital volume.

 

TAU student Michal Ferenz. Photo: Yoram Reshef.

Soffer and her team set up a technical support hotline for online learning; they received as many as 700 calls per day. In addition to assisting professors in overcoming the technicalities of online teaching, the team also created more than 50 video guides showing lecturers how to use online learning tools to make lessons more engaging.

The team also conducted large-scale surveys among 7,000 students and 750 faculty members. They found that a vast majority of students wanted to incorporate online learning into their studies in the future.

Like other universities around the world, TAU also faced the new challenge of conducting online exams and evaluations. Spring semester exams were conducted from home with supervisors overseeing students through Zoom.

During the 2020-2021 academic year, TAU is introducing a pilot computerized authentication system for online exams. The new technology will secure online exams by verifying students’ identity and monitoring their presence and activities during the exam. Although this is a big step forward, Soffer is aware that in the long run adopting more of these technologies may be intrusive. Instead of relying on anti-cheating applications, Soffer says, the University should also encourage alternative evaluation methods, such as essays and group projects.

“The Corona crisis profoundly disrupted higher education and forced it to make the transition to the digital world—and, in a way, I believe this is exactly the kind of disruption that was needed. The question is, how do we move forward from here?” Soffer says.

Innovating on all Levels

“Universities all around the world understood a long time ago that they have to transform learning and to enhance their online and digital tools,” says Yuval Shreibman, Director of TAU Online – Innovative Learning Center.  The Center started producing online courses long before Corona to make academia more accessible through technology.

“COVID-19 caused us to leap forward and address problems that we could previously overlook. At the same time, it shows us that we need to make complementary classroom learning more active and engaging.”

Given the volatile reality and constantly changing regulations, TAU prepared for all possible scenarios for the new academic year. While it intended to offer first-year students the option to physically attend classes, studies were conducted online for the duration of the first semester. In response, Virtual TAU has launched an unprecedented effort to arm lecturers with versatile presentation tools and introduce additional courses that are fully online.


Virtual TAU Team. Photo: Yoram Reshef.

Admissions to the University are also going online, with a new admissions track based on participation and success in specific online courses chosen by each faculty. The new track is currently intended for candidates who, because of COVID-19, could not take standardized university admissions tests. Yet, it also provides greater access to the University for young Israelis from disadvantaged backgrounds or outlying communities, who otherwise might not be able to study at TAU.

In the fall of 2021, TAU plans to launch a new fully online international MBA program, the first of its kind to be offered by an Israeli university. It will combine video courses that students will watch independently, with personal guidance from teaching staff, online study forums and projects. Based on the same high entrance requirements as the regular MBA programs at TAU’s Coller School of Management—recently ranked as the 13th school in the world for producing VC-backed entrepreneurs—the program is expected to attract ambitious students from across the globe.

COVID-19 underlined the importance of online learning at TAU so much that President Ariel Porat created a new position to oversee educational innovation; Prof. Liat Kishon-Rabin became Dean of Innovation in Learning and Teaching in July. “TAU has always prided itself as a leader in educational innovation, but the Corona pandemic has highlighted the need to focus on this field even more,” says Prof. Porat. “I trust that Prof. Kishon-Rabin will build on our existing achievements and lead us through the post-Corona era with vision and success.”

Read about Minducate, an innovation and learning center at TAU. 

Providing Critical Support during Online Learning

Alberto Meschiany. Photo: Moshe Bedarshi.

Despite the positive insights gleaned about online learning, TAU must take into account students who struggled with remote learning as it prepares for a new academic year in the shadow of COVID-19. Alberto Meschiany, Head of the Psychological Services Unit at TAU’s Student Services Division, says that at the beginning of the crisis, his unit experienced a 15% rise in requests for psychological support.

“For many students, the anxiety resulting from the pandemic itself and its economic implications was coupled with the stress of having to study and take exams from home,” he says. “For students who live in the dorms or come from lower socio-economic levels this was exceptionally difficult. Many of them don’t have a quiet place to study. Some live in remote towns that don’t have the Internet network to support continuous online studies.”

Yet, according to Meschiany, it isn’t only the logistical and technological barriers that made the shift to online learning difficult for many TAU students. “Distance from other students can create feelings of alienation and loneliness. All the technology in the world cannot replace the support that students get from their peers,” he says. “In addition, the lack of a personal lecturer-student relationship has a negative effect on academic development. The ability to knock on a lecturer’s door and ask a question or discuss a topic spontaneously is lost with online learning.”

Meschiany believes that as the University adopts more online learning methods, it should make an effort to tailor them to accommodate students with various difficulties. “They will need our active help,” he says.

The Student Viewpoint

Looking back at lessons learned from the “first wave” of online learning, there is no question that TAU can learn the most from its students. Jonathan Berkheim, a master’s student in chemistry and spokesperson for TAU’s Student Union when the pandemic started, experienced the lockdown and its aftermath from several perspectives.

As a senior member of the Student Union, he fielded numerous calls from students who struggled to study within the new framework. Even students who fared well felt shortchanged, according to Berkheim. “The social interaction, class discussions and campus life are crucial parts of the package that students expect from university studies.” 

Jonathan Berkheim. Photo: Moshe Bedarshi.

At the same time, Berkheim says that the unusual circumstances broke traditional, hierarchical barriers between students and professors. They found themselves communicating directly on WhatsApp groups, saw each other’s homes during Zoom sessions, and shared similar experiences of life during the lockdown. “I hope that the University will embrace this new paradigm for student-professor relations in the future.”

In addition, as a teaching assistant, he experienced distance learning from the other side of the virtual podium: “Something gets lost in translation. Students get distracted more easily. It was hard for me to know if they really understood what I was teaching.”

Finally, as a student himself, he found that watching recorded lessons at his own pace was convenient. “Face-to-face learning in the classroom is crucial, but combining it with independent online studies will have great benefits for students,” Berkheim concludes.

Among TAU students studying remotely are also hundreds of international students from over 100 countries, who are enrolled in over 60 English-led academic programs offered by TAU International. In the midst of the crisis, TAU International launched an online summer course, titled: “COVID-19: From Crisis to Opportunity,” which attracted more than 80 participants from Asia, South America, North America and Europe.

Read about how TAU Impact, the University’s flagship community service program, adapted to the pandemic.
 

As TAU heads toward another academic year, it is clear that life with COVID-19 has become the new normal. All players involved in online learning understand that TAU must embrace the advantages moving forward.

“Until recently, when I was presenting my own field of research—which deals with future trends in the labor market and predicts that people would increasingly shift to working from home—people would tell me that it sounds too futuristic,” says Prof. Mundlak. “Now it is has become a reality. The future is here.”

Featured image: TAU Life Sciences Prof. Nir Ohad films a remote lecture at the TAU Online studio. Photo: Yoram Reshef.

TAU Scientists Develop Innovative Therapy to Prevent Deafness

The treatment is inserted into the ear, prevents hearing loss caused by a genetic mutation.

A new study at Tel Aviv University presents an innovative treatment for deafness, based on the delivery of genetic material into the cells of the inner ear. The genetic material ‘replaces’ the genetic defect and enables the cell to continue functioning normally.

The scientists were able to prevent the gradual deterioration of hearing in mice with a genetic mutation for deafness. They maintain that this novel therapy could lead to a breakthrough in treating children born with various mutations that eventually cause deafness.

The study was led by Prof. Karen Avraham and Shahar Taiber, a student in the combined MD-PhD track, from the Department of Human Molecular Genetics and Biochemistry at the Sackler Faculty of Medicine, and the Sagol School of Neuroscience, and Prof. Jeffrey Holt from Boston Children’s Hospital and Harvard Medical School. Additional contributors included Prof. David Sprinzak from the School of Neurobiology, Biochemistry and Biophysics at the George S. Wise Faculty of Life Sciences at Tel Aviv University. The paper was published in EMBO Molecular Medicine.

Deafness is the most common sensory disability worldwide. According to the World Health Organization there are about half a billion people with hearing loss around the world today, and this figure is expected to double in the coming decades. One in every 200 children is born with a hearing impairment, and one in every 1,000 is born deaf. In about half of these cases, deafness is caused by a genetic mutation. There are currently about 100 different genes associated with hereditary deafness. 

Prof. Avraham: “In this study we focused on genetic deafness caused by a mutation in the gene SYNE4 – a rare deafness discovered by our lab several years ago in two Israeli families, and since then identified in Turkey and the UK as well. Children inheriting the defective gene from both parents are born with normal hearing, but gradually lose their hearing during childhood. This happens because the mutation causes mislocalization of cell nuclei in the hair cells inside the cochlea of the inner ear, which serve as soundwave receptors and are thus essential for hearing. This defect leads to the degeneration and eventual death of hair cells.” 

Shahar Taiber: “We implemented an innovative gene therapy technology: we created a harmless synthetic virus and used it to deliver genetic material – a normal version of the gene that is defective in both the mouse model and the affected human families. We injected the virus into the inner ear of the mice, so that it entered the hair cells and released its genetic payload. By so, we repaired the defect in the hair cells, and enabled them to mature and function normally.”

The treatment was administered soon after birth and the mice’s hearing was then monitored using both physiological and behavioral tests. Prof. Holt: “The findings are most promising: Treated mice developed normal hearing, with sensitivity almost identical to that of healthy mice who do not have the mutation”. Following the successful study, the scientists are currently developing similar therapies for other mutations that cause deafness.

Prof. Wade Chien, MD, from the NIDCD/NIH Inner Ear Gene Therapy Program and Johns Hopkins School of Medicine, who was not involved in the study, illuminates its significance: This is an important study that shows that inner ear gene therapy can be effectively applied to a mouse model of SYNE4 deafness to rescue hearing. The magnitude of hearing recovery is impressive. This study is a part of a growing body of literature showing that gene therapy can be successfully applied to mouse models of hereditary hearing loss, and it illustrates the enormous potential of gene therapy as a treatment for deafness.

The study was supported by the BSF – US-Israel Binational Science Foundation, the NIH – National Institutes of Health, the ERC – European Research Council, and the Israel Precision Medicine Partnership Program of the Israel Science Foundation.

Two TAU Professors Win 2020 Nature Mentoring Award

Prof. Neta Erez and Prof. Tal Pupko, nominated by students, are building the future generation of scientists.

Two scientists from Tel Aviv University – Professor Neta Erez, head of the Department of Pathology at Tel Aviv University’s Sackler School of Medicine, and Professor Tal Pupko, head of the Shmunis School of Biomedicine and Cancer Research at the Life Sciences Faculty, have won the 2020 Nature Research Awards for Mentoring in Science, given by the Springer Nature Group, which is the home of the leading journal Nature.

The prestigious award (which is given in a different country each year), was given in Israel this year, with Tel Aviv University sweeping all the honors for mid-career mentoring. The award is given to scientists who excel in mentoring research students in their laboratories, thus contributing to the development of the future of science — in Israel in particular and in the world in general. Both winners will share the $10,000 prize. They said that the prize was especially moving for them because the ones who had nominated them for it were the very ones whom they mentored — the students and graduates of their laboratories.

Professor Erez, who established a laboratory ten years ago for researching metastasis of breast cancer and melanoma, and who has mentored 16 doctoral candidates and five master’s degree students so far, said, “For me, mentoring is a central part of my identity as a scientist. When a doctoral candidate comes to me, I tell them: ‘You are starting off as my student, and I want you to end up as my peer.’ For that reason, my role as a mentor is not only to accompany the research. My role is to teach my students to think and do research like scientists, and to find their own way in science and in life in general.  I am very proud of their accomplishments. Quite a few graduates of the laboratory have been awarded prizes and grants. As of now, four of the students have completed their medical studies and are planning to combine medicine and research. One is a research fellow and a lab manager in an academic setting, another is doing post-doctoral work in the United States, and four others are working as scientists in the biotech industry. In addition, I serve as a mentor for two young researchers who recently established their own laboratories.” 

Professor Pupko, who established a laboratory 17 years ago that deals with molecular evolution and bioinformatics, has mentored 18 doctoral candidates so far. “The members of the academic staff are evaluated based on a variety of parameters: research grants, publications and teaching. Another index, which I feel does not receive enough emphasis, is the success of a staff member’s laboratory graduates — the young scientists whom he taught, mentored, and ‘raised.'” I invest a great deal of thought and effort in my students in order to support, encourage, advise, and nurture them. All 12 doctoral candidates who completed their degree in my laboratory have gone on to do post-doctoral work.  Four of them are staff members in academia (including three at Tel Aviv University) — a particularly high number for an academic research laboratory. Other graduates of my laboratory hold high-ranking positions in the hi-tech and bio-tech industries. As I see it, a student who excels is better than another three scientific papers. My aim is to raise up generations of researchers in Israel. I see that as my mission.”

The prize committee, which included Professor Karen Avraham of the Faculty of Medicine at Tel Aviv University, announced that it had chosen the two recipients because “it was impressed with their contagious enthusiasm of former students,” who had nominated them for the award. The committee also praised Professor Pupko for his inclusive approach and encouragement of a healthy work-life balance alongside professional excellence, and Professor Erez for her work to advance women in science and for projects that bring her influence as a mentor to wider circles, including ones outside her laboratory.

TAU Study Proves that Light Can Kill Coronavirus

Groundbreaking research finds UV-LED diodes efficiently and cheaply disinfect social spaces.

A revolution in disinfection? Researchers from Tel Aviv University have proven that the coronavirus can be killed efficiently, quickly and cheaply using ultraviolet (UV) light-emitting diodes (UV-LEDs). This is the first study in the world conducted on the disinfection efficiency of a virus from the family of coronaviruses using UV-LED irradiation at different wavelengths or frequencies. The study was led by Prof. Hadas Mamane, Head of the Environmental Engineering Program at the School of Mechnical Engineering, Iby and Aladar Fleischman Faculty of Engineering, and was conducted in collaboration with Prof. Yoram Gerchman of Oranim College, Dr. Michal Mandelboim, the Director of the National Center for Influenza and Respiratory Viruses at Sheba Medical Center at Tel HaShomer, and Nehemya Friedman from Tel Hashomer. The article was published in the Journal of Photochemistry and Photobiology B: Biology.

In the study, the researchers tested the optimal wavelength for killing the coronavirus, and found that a length of 285 nanometers was almost as efficient in disinfecting the virus as a wavelength of 265 nanometers, requiring less than half a minute to destroy more than 99.9% of the coronaviruses. This result is significant because the cost of 285 nm LED bulbs is much lower than that of 265 nm bulbs, and the former are also more readily available. Eventually, as the science develops, the industry will be able to make the necessary adjustments and install the bulbs in robotic systems, or air conditioning, vacuum, and water systems, and thereby be able to efficiently disinfect large surfaces and spaces. Prof. Mamane believes that the technology will be available for use in the near future.

“The entire world is currently looking for effective solutions to disinfect the coronavirus,” says Prof. Mamane. “The problem is that in order to disinfect a bus, train, sports hall or plane by chemical spraying, you need physical manpower, and in order for the spraying to be effective, you have to give the chemical time to act on the surface. We know, for example, that medical staff do not have time to manually disinfect, say, computer keyboards and other surfaces in hospitals – and the result is infection and quarantine. The disinfection systems based on LED bulbs, however, can be installed in the ventilation system and air conditioner, for example, and sterilize the air sucked in and then emitted into the room.”

“We discovered that it is quite simple to kill the coronavirus using LED bulbs that radiate ultraviolet light,” explains Prof. Mamane. “But no less important, we killed the viruses using cheaper and more readily available LED bulbs, which consume little energy and do not contain mercury like regular bulbs. Our research has commercial and societal implications, given the possibility of using such LED bulbs in all areas of our lives, safely and quickly. Of course, as always when it comes to ultraviolet radiation, it is important to make it clear to people that it is dangerous to try to use this method to disinfect surfaces inside homes. You need to know how to design these systems and how to work with them so that you are not directly exposed to the light.”

Ultraviolet radiation is a common method of killing bacteria and viruses, and most of us are familiar with such disinfecting bulbs from their use in water purifiers, such as Tami4. UV radiation mainly damages nucleic acids. Last year, a team of researchers led by Prof. Mamane and Prof. Gerchman patented a combination of different UV frequencies that cause dual-system damage to the genetic load and proteins of bacteria and viruses, from which they cannot recover-which is a key factor that is ignored.“ In the future, we will want to test our unique combination of integrated damage mechanisms and more ideas we recently developed on combined efficient direct and indirect damage to bacteria and viruses on different surfaces, air and water.”

Featured image: Prof. Hadas Mamane

COVID-19 Takes TAU’s Legal Clinics into High Gear

Clinic staff share experiences of protecting those most affected by pandemic

Dr. Yuval Livnat, Adv. Anat Ben Dor and Adv. Irina Rozina

The Refugee Rights Clinic

The coronavirus crisis forced the Clinic to shift to remote work. However, this style of working is not ideal for asylum-seekers, and significant challenges arose. Intake interviews with new clients are complex; they touch on sensitive issues and may require translation services. The absence of face-to-face interviews makes it hard to build trusting relationships, and the completion and transfer of required forms and documentation is difficult; protecting personal privacy and information is problematic when communication takes place over the telephone or on Zoom. During this period, the refugee and asylum-seeker community faces a major economic crisis. Israeli citizens are supported by a social safety net. Asylum-seekers are not eligible for this support and have legitimate fears that they will be unable to pay rent and feed their children. In light of this harsh reality, April’s Supreme Court ruling cancelling the “Deposit Law” (authorizing the expropriation of 20% of an asylum seeker’s monthly earnings) provided some relief. This decision came three years after the Clinic filed the Supreme Court petition arguing that the deposit violated the workers’ legal rights, in cooperation with Kav LaOved. The Supreme Court ordered that funds be returned. The Clinic’s students, along with volunteers from Kav LaOved, helped asylum-seekers to complete the required forms and open bank accounts, so their deposits could be returned.”

Adv. Liad Strolov and Adv. Yael Havassy

The Clinic for the Rights of Holocaust Survivors and the Elderly

The coronavirus crisis has significantly impacted our clinic. Firstly, the way we work with clients and students changed entirely after we transitioned to online rather than in-person meetings. This is particularly challenging for older clients that are unfamiliar with Zoom and other technologies. In addition, since the elderly population faces the greatest risk from the coronavirus, many are suffering from side issues caused by the crisis such as intense loneliness; inaccessibility to caregiver services; threats of eviction from nursing facilities due to an increased demand for space to care for COVID-19 patients; restrictions on movement in public housing facilities; and of course, restrictions barring them from returning to work under the current emergency regulations.”

Adv. Irit Ulman and Adv. Idit Zimmerman

The Workers’ Rights Clinic

At the onset of the pandemic, it was clear that regulations regarding labor law and social security would be greatly affected. Israel opted to deal with the widespread closure of workplaces by increasing and extending unemployment benefits. As the number of unemployed Israelis continued to rise, those of us working in the field were occupied with unprecedented challenges to labor law resulting from the crisis. From the outset, the Clinic collaborated with various organizations to ensure that workers’ rights were protected. In early April, the government passed emergency regulations eliminating the legal requirement for employers to obtain a permit prior to sending female employees under protection (e.g. during pregnancy, maternity leave, etc.) on furlough. We submitted an urgent petition to the Supreme Court on behalf of a group of organizations, demanding that the regulations be repealed; the petition argued that the process by which the regulations were passed were flawed, and that the regulations caused disproportionate harm to women. Following the petition’s submission, and prior to a hearing at the Supreme Court, the regulations were annulled.

The crisis also created increased challenges for foreign caregivers working with the elderly, due to severe movement limitations. Since there is an increased risk to the elderly, family members often placed severe restrictions on the movements of in-home caregivers. Although their fears may have been warranted, severe restrictions could result in infringements on human rights and workers’ rights (e.g. freedom of movement, loss of days off, etc.). The Clinic, in cooperation with the Clinic for the Rights of Holocaust Survivors and the Elderly, appealed to policy-makers to ensure that the rights of caregivers and the elderly people they support were protected.”

Dr. Eran Tzin and Adv. Amnon Keren

The Clinic for Environmental Justice and the Protection of Animal Rights

“At the beginning of the crisis, the Clinic offered support for animal welfare organizations and activists struggling with challenges arising from the imposed lockdown; the Clinic helped to map crucial issues, formulate strategies, and provided legal advice and representation, as needed. A key aspect of the Clinic’s activities was ensuring ongoing routine care of animals in various facilities (e.g. sanctuaries, shelters, etc.) since severe restrictions on activity and movement limited the possibility of providing them with food and vital veterinary care. The Clinic appealed to the Ministry of Health and other relevant parties, requesting that those caring for animals be exempted from emergency regulations, including in areas heavily affected by the virus. Furthermore, we requested that measures preventing harm to animals be an integral part of any national plan to deal with the crisis. Our efforts, in combination with public pressure, were successful and led to the requested policy changes. The Clinic also succeeded in canceling fines given to individuals while caring for animals, despite their compliance with emergency regulations. These are concerned and caring citizens who volunteered and used their own funds to care for animals, even though they faced financial difficulties due to the crisis.”

Featured image: Human Rights Clinic students and attorneys, Hicham Chabaita and Debby Tal Sadeh.

Lack of Teacher Support during Pandemic Causes Acute Emotional Harm

TAU study provides insights into preventing burnout among educators.

A new Tel Aviv University study led by Dr. Shahar Lev-Ari, Head of the Department of Health Promotion at TAU’s Sackler Faculty of Medicine study examined the psychological resilience of teachers before and during the coronavirus pandemic. The researchers monitored two groups of teachers in central Israel through the greater part of a single schoolyear. The first group received professional support (via the IBSR method), which included workshops and tools for promoting personal health, relieving stress and strengthening mental resilience, while the control group continued to work as usual in class and then in online sessions, without this support.

The study took place from November 2019 to May 2020, with participants teaching first in the classroom and then, starting with Israel’s first lockdown in March 2020, exclusively online.  In a questionnaire handed out before the beginning of the first study, teachers reported high levels of burnout as a result of large classes, schedule overload and lack of satisfactory resources.

The research team’s findings indicate significant gaps: On one hand, teachers in the group that received psychological support reported a significant rise in mental resilience and satisfaction with their lives in general, which continued after the onset of the pandemic. During the pandemic, they reported a better ability to cope and an improvement in their emotional welfare, including more positive emotions, a stronger sense of connection to their work and purpose, and greater drive and ambition. They also reported enhanced ability to relate to and listen to their students and to maximize their professional capabilities in class.

On the other hand, the control group, which did not receive support, reported that feelings of frustration and burnout, exhaustion and low self-fulfillment intensified, both during the academic year and the pandemic, when online teaching was required. The teachers in this group reported feeling “total exhaustion” at the end of the day, and sometimes also frustration and a lack of motivation to start a new workday.

The study was conducted by: Dr. Shahar Lev-Ari, research student Tsafnat Zadok from the Department of Health Promotion, Dr. Ronit Jakobovich, Etti Dvash and Keren Zafrani. The workshops were led by Keren Zafrani, a professional teacher and IBSR expert.

Dr. Shahar Lev-Ari: “The pandemic posed new challenges that naturally generated feelings of stress and anxiety among teachers. In addition to the quick transition to online teaching, teachers had to cope with uncertainty and constantly changing regulations, as well as personal fear of contracting the virus.

Our study clearly shows that when mental resilience is prioritized and tools for overcoming their stress and anxiety are provided, a rise in motivation and emotional welfare is observed. Accordingly, we observed that when teachers did not receive the required guidance and mental skills, their negative feelings, which were also reported in normal times, grew and intensified. This was especially acute during the pandemic – reaching levels of extreme exhaustion and a lack of motivation to start the workday.”

Dr. Lev-Ari adds: “Many studies have shown that teachers’ burnout is a covert cause of heart disease and sleep disorders, and also has a negative impact on the immune system. Burnout is also the reason why many teachers leave the profession after just a few years of teaching. I hope that following the significant improvement exhibited in this study, the education system will implement intervention programs based on the model described above or similar models. This is especially critical during the pandemic, when teachers face new pressures that intensify feelings of stress, anxiety and frustration.”

New Discovery: Development of the Inner Ear in Embryos is Similar to Crystal Formation

This discovery could contribute to the development of treatments for hearing loss based on regeneration of hair cells in the ear.

An interdisciplinary study headed by Prof. David Sprinzak, a researcher from the School of Neurobiology, Biochemistry and Biophysics at the George S. Wise Faculty of Life Sciences at Tel Aviv University, showed for the first time that physical forces are involved in the development of the inner ear in mammalian embryos.

Prof. Sprinzak: “We identified a new developmental mechanism that is driven by mechanical forces: the organization of hair cells in the inner ear resembles the way atoms are organized into a crystal. This is a revolutionary finding that changes fundamental perceptions in the field of developmental biology.”

The study was performed by Roie Cohen and Liat Amir-Zilberstein of Prof. Sprinzak’s laboratory; Prof. Karen Avraham and Shahar Taiber of the Sackler Faculty of Medicine; and other researchers from the Faculty of Exact Sciences and from the Sagol School of Neuroscience at Tel Aviv University. Researchers from Switzerland, and Japan also participated in the study. The paper describing the work was published in the prestigious journal, Nature Communications, in October 2020.

Prof. Sprinzak explains: “The mammalian ear has three parts: the outer, middle, and the inner ear. Within the inner ear, there is a spiral structure, the cochlea, which contains sensory cells called hair cells. Hearing occurs when sound waves entering the inner ear cause the tiny hairs located on the hair cells to vibrate. These vibrations are then converted into electrical signals that are transmitted to the brain.

The hair cells in the cochlea are arranged in a highly organized pattern, where the hair cells and a second cell type called supporting cells form an alternating checkerboard-like pattern. This organization is important since different regions along the cochlea respond to different sound frequencies. Such a remarkably organized cellular system is rather uncommon in nature – in fact, the inner ear is one of the most organized tissues in the mammalian body. In this work, we investigated the mechanism that causes the hair cells to arrange in such a pattern during embryonic development. To do so, we conducted an interdisciplinary study that involved two innovative approaches: a new imaging technology and computational simulations of the process.”

 

In order to track the development of hair cells in the embryo, the researchers studied mouse embryos at different developmental stages. They found that early on, the cells in the tissue are disordered and undifferentiated; namely, their type and position was not determined yet. As development progresses, the cells differentiate into hair cells and supporting cells. Then, they gradually rearrange into the organized checkboard-like pattern.

Prof. Sprinzak: “Until now, most researchers in the field focused on the process of cell differentiation, which is controlled by intercellular communication. We hypothesized that this was not enough to explain the observed behavior, and we decided to examine how cells rearrange to form an ordered pattern after differentiation.” In order to do so, the researchers developed a new imaging technology that is based on three-dimensional time lapse imaging of the inner ear using a specialized microscopy setup. This approach allowed generating time-lapse videos of the development of the tissue and to track the morphological processes occurring over several days.

Prof. Sprinzak: “This is the first time that the process has been observed continuously and at high resolution. We observed that the initially disordered hair cells and supporting cells actively move until they gradually arrange into an ordered array. Neighboring cells, known as Hensen cells, move in one direction, exerting shear forces on the hair cells – forces that act in parallel to the layer of cells. These forces squeeze the hair cells together, causing them to arrange in a compact and organized pattern.”

In the next stage, the researchers used computer simulations to model the patterning process. The model showed that two main mechanical forces acted on the hair cells during the patterning process: shear forces, which caused squeezing and movement of the hair cells within the tissue, and repulsion forces between the hair cells, which keep the hair cells from getting too close to one another. Prof. Sprinzak: “We were surprised to discover that the patterning process of the hair cells in the cochlea highly resembled a well-known physical process – the patterning of atoms during the formation of a crystal. Just as atoms form a highly ordered crystal when external forces are exerted on them, so the hair cells and the supporting cells rearrange into a highly ordered pattern in response to mechanical forces acting on them. This is a completely new way of thinking in the field of developmental biology. The insights obtained from our study shed light on new research directions relevant for many other developmental processes in other organs.”

Prof. Lawrence Lustig, the Howard Smith Professor and Chair of Otolaryngology at Columbia University and New York Presbyterian Hospital who was not involved in the research, added that the study’s findings could also have significant medical implications. “All the hair cells in our inner ear are created at an embryonic stage and do not regenerate during one’s lifetime. Death of hair cells in the inner ear at any stage of life leads to permanent hearing loss. In recent years, a lot of effort have been made by the scientific community to develop therapeutic approaches to hearing loss based on regeneration of hair cells – a process where formation of new hair cells is induced by genetic therapy or small signaling molecules. This study makes an important contribution to understanding the process of hair cell regeneration and is a critical step towards this goal.”

Featured Image: Prof. David Sprinzak

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